Prevention of internal initiation

Details Prevention of internal initiation Prevention of internal initiation

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C07K 14/34 (2006.01) C07K 14/55 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2063799

2063799 9101374 PCTABS00003 Described is a method of preventing undesired initiation of translation at an internal initiator codon in a DNA sequence encoding a polypeptide, wherein a Shine-Dalgarno sequence is located upstream from the internal initiator codon. The method includes altering either the internal initiator codon or the Shine-Dalgarno sequence, or both, such that inappropriate initiation of translation and/or ribosome binding is prevented.

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