Primer walking cycle sequencing

Details Primer walking cycle sequencing Primer walking cycle sequencing

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07B 61/00 (2006.01)

Patent

CA 2224396

The invention provides a method for primer walking sequencing of nucleic acid wherein a presynthesised set of walking primers is used, the said primers having a raised annealing temperature and/or improved annealing properties without increasing sequence complexity.

L'invention se rapporte à un procédé de séquençage progressif d'une amorce d'acide nucléique dans lequel est utilisé un ensemble présynthétisé d'amorces progressives, ces amorces ayant une température de recuit élevée et/ou de meilleures propriétés de recuit sans accroître la complexité de la séquence.

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