Primers and method of detecting bacteria

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/00 (2006.01) C07K 14/245 (2006.01) C07K 14/255 (2006.01) C07K 14/28 (2006.01) C07K 14/31 (2006.01) C07K 14/32 (2006.01) C12Q 1/02 (2006.01) C12Q 1/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2463689

The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

L'invention concerne une première amorce pouvant amplifier de manière détectable les ADN codant les ARN 16Sr de bactéries appartenant aux genres Escherichia, Salmonella et Vibrio lorsqu'elle est soumise à une PCR dans des conditions déterminées, mais n'amplifiant pas les ADN codant les ARN 16Sr de chloroplaste et les ADN codant les ARN 16Sr de mitochondrie lorsqu'elle est soumise à une PCR dans les mêmes conditions. L'invention concerne également une deuxième amorce pouvant amplifier de manière détectable les ADN codant les ARN 16Sr de Staphylococcus aureus et de Bacillus cereus lorsqu'elle est soumise à une PCR dans des conditions déterminées, mais n'amplifiant pas les ADN codant les ARN 16Sr de chloroplaste et les ADN codant les ARN 16Sr de mitochondrie lorsqu'elle est soumise à une PCR dans les mêmes conditions.

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