C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01)
Patent
CA 2217594
The present invention relates to a DNA amplification primer pair for the simultaneous amplification of multiple highly polymorphic genomic loci, which comprises R14B264/Q560mak (SEQ ID NO:1/SEQ ID NO:2). A method for the DNA fingerprinting identification of genetically related or unrelated individuals, which comprises the steps of: a) collecting genomic DNA sample of the individuals; b) performing DNA amplification of the DNA samples of step a) using the primer pair R14B264/Q560mak (SEQ ID NO:1/SEQ ID NO:2); and c) separating the amplified DNA segments of step b); whereby three markers of the genomic DNA of different size are amplified and serve as DNA fingerprinting of the individuals.
La présente invention concerne une paire d'amorces d'amplification d'ADN, pour l'amplification simultanée de loci génomiques multiples hautement polymorphes, qui comprennent les R14B264/Q560mak (n· ID SEQ: 1/n· ID SEQ: 2). L'invention concerne aussi un procédé d'identification, par l'empreinte protéique d'ADN, de personnes parentes ou non sur le plan génétique, procédé comprenant les étapes suivantes: a) recueillir un échantillon d'ADN génomique des personnes en question; b) effectuer l'amplification d'ADN sur les échantillons de l'étape a), en utilisant la paire d'amorces R14B264/Q560mak (n· ID SEQ: 1/n· ID SEQ: 2); c) séparer les segments d'ADN amplifiés de l'étape b); trois marqueurs de l'ADN génomique de différentes tailles sont amplifiés et servent d'empreinte protéique d'ADN des personnes considérées.
Labuda Damian
Melancon Serge Bernard
Tang Jianqing
Vanasse Michel
Centre de Recherche de L'hopital Sainte-Justine
Ogilvy Renault
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