Primers and process for detecting human papillomavirus...

Details Primers and process for detecting human papillomavirus... Primers and process for detecting human papillomavirus...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2074069

The invention relates to primers and a method of detecting human papilloma virus (HPV) genotypes by means of the Polymerase Chain Reaction (PCR), The invention provides such primers and such PCR conditions that in principle any genital HPV genotype is detected. The general primers according to the invention consist of an oligonucleotide which comprises at the 3' end one of the sequences (I), or a sequence which is homologous to one of these four sequences as to at least 75 %. In order to obtain amplification even in the case of a limited number of mismatches, in the PCR a relatively low annealing temperature of 30-50 °C and a relatively high Mg2+ concentration of 2-10 mM are used, The invention enables a sensitive and reliable preselection of samples to be examined, such as cervical smears.

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