Primers for the detection of hiv-1

Details Primers for the detection of hiv-1 Primers for the detection of hiv-1

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2222769

The present invention provides improved primers for the polymerase chain reaction (PCR) amplification of a nucleic acid sequence from the gag gene of the human immunodeficiency virus type 1 (HIV-1). The primers and amplification methods of the invention enable the detection of all HIV-1 group M isolates with nearly uniform efficiency.

L'invention fournit des amorces améliorées pour l'amplification par la réaction en chaîne de la polymérase (PCR) d'une séquence d'acides nucléiques du gène gag du virus de l'immunodéficience humaine de type 1 (VIH-1). Les amorces et les méthodes d'amplification dévoilées permettent de détecter tous les isolats du groupe M du VIH-1 avec une efficacité presque uniforme.

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