Prion elisa

Details Prion elisa Prion elisa

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2684798

Assays for detecting PrPSc in a sample are described. In particular, pathogenic prion ELISAs are described. The assays utilize pathogenic prion-specific reagents to capture the PrPSc and digestion with a site-specific protease, for example trypsin or SV-8 protease, to reduce the amount of interference from non-pathogenic prion proteins that are occasionally present in the samples.

Cette invention concerne des dosages permettant de détecter PrPSc dans un échantillon. Plus particulièrement, cette invention concerne les techniques de dosages immuno-enzymatiques (ELISA) de la protéine du prion pathogène. Ces techniques de dosages immuno-enzymatiques consistent à utiliser des agents réactifs propres au prion pathogène pour capturer le PrPSc et à utiliser la digestion avec une protéase propre à un site, par exemple la trypsine ou SV-8 protéase, pour réduire la quantité d'interférence provenant des protéines du prion non pathogènes contenues occasionnellement dans les échantillons.

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