Probe and method for detecting yeasts of species candida krusei

Details Probe and method for detecting yeasts of species candida krusei Probe and method for detecting yeasts of species candida krusei

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 1/16 (2006.01)

Patent

CA 2175227

The detection and characterisation of yeasts belonging to species Candida krusei is disclosed. A specific probe, in particular a DNA probe for detecting and, in particular, identifying species Candida krusei and typing (infra-specific characterisation) Candida krusei strains, is particularly disclosed. The expected results are achieved by means of a probe selected from the following genetic (or related) tools: at least one portion of at least one DNA and/or RNA fragment (F) with a size of 7-4 kb, said fragment being specifically hybridisable with the DNA and/or RNA of Candida krusei while not coding for rRNA; at least one portion of the transcription and/or translation products of (F); and a combination of the above-mentioned tools. The probe may be used in medical diagnostics and industrial therapeutical tests.

La présente invention a trait à la détection et la caractérisation de levures appartenant à l'espèce Candida krusei. En particulier, elle fournit une sonde spécifique, notamment nucléique, pour la détection et, en particulier, l'identification de l'espèce Candida krusei et le typage (caractérisation infra-spécifique) des souches de l'espèce Candica krusei. Les performances attendues, à cet égard, sont atteintes par la sonde qui est choisie parmi les outils génétiques (ou apparentés) suivants: au moins une partie d'au moins un fragment F d'ADN et/ou ARN, de taille comprise entre 7 et 4 kb, hybridant spécifiquement avec l'ADN et/ou l'ARN de Ccndida krusei et ne codant pas pour de l'ARNr; au moins une partie des produits de transcription et/ou de traduction de F; et une association des susdits outils. Application; diagnostic médical, contrôles industriels thérapeutiques.

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