Probe arrays for detecting multiple strains of different...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2582137

The present invention provides probe arrays and methods of using the same for concurrent and discriminable detection of multiple strains of different species. In one aspect, the probe arrays of the present invention are nucleic acid arrays comprising (1) a first group of probes, each of which is specific to a different respective strain of a first species; and (2) a second group of probes, each of which is specific to a different respective strain of a second species. In many embodiments, the nucleic acid arrays of the present invention further include a third group of probes, each of which is specific to a different strain of a third species. In one example, a nucleic acid array of the present invention includes probes for sequences selected from SEQ ID NOs: 1 to 18,598, and can discriminably detect different strains of Streptococcus pyogenes, Streptococcus agalactiae and Staphylococcus epidermidis.

L'invention concerne des ensembles de sondes ainsi que leurs procédés d'utilisation permettant de détecter simultanément et de manière discriminante plusieurs souches de différentes espèces. Dans un aspect, les ensembles de sondes sont des ensembles d'acides nucléiques comprenant (1) un premier groupe de sondes, chacune étant propre à une souche correspondante différente d'une première espèce; et (2) un second groupe de sondes, chacune étant propre à une souche correspondante différente d'une seconde espèce. Dans plusieurs modes de réalisation, les ensembles d'acides nucléiques comportent en outre un troisième groupe de sondes, chacune étant propre à une souche différente d'une troisième espèce. Dans un exemple, un ensemble d'acides nucléiques comporte des sondes pour des séquences choisies dans SEQ ID NO: 1 à 18 598 et peut détecter d'une manière discriminante différentes souches de Streptococcus pyogenes, Streptococcus agalactiae et Staphylococcus epidermidis.

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