Probe for constructing probe polymer, method of constructing...

Details Probe for constructing probe polymer, method of constructing... Probe for constructing probe polymer, method of constructing...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C07K 14/00 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2387755

The present invention provides a method for measuring a target gene under isothermal conditions without using enzyme. A pair of probes each having n (n >=3) base sequence regions complementary to each other are hybridized alternately to form a double-stranded probe-polymer. A base pair at branched sites of each complementary base sequence region is designed to be a G (guanine)-C (cytosine) bond, whereby a stable double-stranded probe-polymer is formed. One of complementary portions in one probe is constituted to have a base sequence complementary to a part of a target gene, whereby a target gene-probe-polymer complex is formed and the target gene is measured.

L'invention concerne un procédé d'analyse d'un gène cible dans des conditions isothermes sans utiliser aucun enzyme. Un polymère sonde bicaténaire est formé par hybridation de plusieurs paires de sondes, chacune consistant en une paire de sondes présentant n (n >/= 3) domaines de séquence de bases complémentaires les uns des autres, de manière à former des croisements alternes. Au point de branchement des domaines de séquence de bases complémentaires, la séquence de bases est liée à un lien G (guanine) C (cytosine) de manière à fabriquer un polymère sonde bicaténaire stable. Un complexe d'un gène cible avec ledit polymère sonde est formé au moyen d'une séquence de bases complémentaire à une partie dudit gène cible, en tant qu'un des domaines complémentaire de la sonde, et, ainsi, ledit gène cible est analysé.

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