Probe for diagnosing of infectious diseases

Details Probe for diagnosing of infectious diseases Probe for diagnosing of infectious diseases

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2147618

The invention provides a probe useful for rapidly detecting and identifying fungi causative of infectious diseases by digesting completely a DNA extracted from a fungus Candidat albicans with EcoRI, inserting the same into an appropriate cloning vector, and picking up a probe containing DNA fragments unique to each fungus. The invention also provides a standard sequence suitable for making reference to a genomic DNA contained in a clinical specimen by elucidating the base sequence of the probe thus picked up.

L'invention concerne une sonde permettant de détecter et d'identifier rapidement des champignons à l'origine de maladies infectieuses, le procédé d'obtention de ladite sonde consistant à faire digérer complètement un ADN extrait d'un champignon Candida albicans avec EcoRI, à introduire celui-ci dans un vecteur de clonage approprié, et à prélever une sonde contenant des fragments d'ADN uniques pour chaque champignon. L'invention permet également d'obtenir une séquence standard convenant pour faire référence à un ADN génique contenu dans un échantillon clinique, par élucidation de la séquence de base de la sonde ainsi prélevée.

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