C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 37/00 (2006.01)
Patent
CA 2471658
A probe medium which comprises a probe capable of binding specifically to a target substance, a substance having a site capable of binding to the probe, and/or a substance having a site capable of binding to the surface of a base material. A substrate having the probe fixed thereto is produced by a method which comprises the step of preparing the substrate and the step of adding the probe medium to the substrate. A DNA array is produced by a process which comprises the step of preparing the base board and the step of adding the probe medium to the substrate. A target substance is detected by a method which comprises the step of preparing the substrate, the step of adding the probe medium to the substrate, and the step of detecting the target substance by using the base material having the probe fixed thereto. The probe medium is produced by a process which comprises the step of preparing the probe, a substance having a site capable of binding to the probe and a substance having a site capable of binding to the surface of the base material, the step of packing solutions respectively containing the substances having the binding sites in containers, and the step of mixing the probe with the substances having the binding sites in the course of fixing the probe on the base material.
La présente invention concerne un matériau de sonde comprenant une sonde capable de se lier spécifiquement à une substance cible, une substance possédant un site capable de se lier à la sonde, et/ou une substance possédant un site capable de se lier à la surface d'un matériau de base. Selon l'invention, on produit un substrat sur lequel est fixé la sonde de la manière suivante : on prépare le substrat et on ajoute le matériau de sonde au substrat. On produit une sonde ADN de la manière suivante : on prépare une carte support et en ajoute le matériau de sonde au substrat. On détecte une substance cible de la manière suivante : on prépare le substrat, on ajoute le matériau de sonde au substrat, et on utilise le matériau de base sur lequel est fixée la sonde pour détecter la substance cible. On produit le matériau de sonde de la manière suivante : on prépare la sonde, on prépare une substance possédant un site capable de se lier à la sonde et une substance possédant un site capable de se lier à la surface du matériau de base, on introduit les solutions respectives contenant les substances possédant les sites de liaison dans des contenants, et on mélange la sonde avec les substances possédant les sites de liaison au moment où l'on fixe la sonde sur le matériau de base.
Iwata Kenichi
Kameyama Makoto
Okada Yoshikatsu
Canon Kabushiki Kaisha
Ridout & Maybee Llp
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