Probe optimization methods

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2572176

The present invention provides methods for optimizing nucleic acid detection assays for use in basic research and clinical research. Specifically, the invention provides a method for empirically optimizing nucleic acid probes by testing them with samples containing genomic DNA with variations in copy number in different regions of the genome. The invention enables the optimization of probes for any hybridization based assay including microarrays, bead-based assays, genotyping assays and RNAi assays.

L'invention concerne des méthodes permettant d'optimiser des essais de détection d'acide nucléique destinés à être utilisés dans la recherche fondamentale et dans la recherche clinique. D'une manière plus spécifique, l'invention concerne une méthode permettant d'optimiser de manière empirique des sondes d'acide nucléique en les testant à l'aide d'échantillons contenant de l'ADN génomique présentant des variations dans le nombre de copies dans différentes régions du génome. L'invention permet d'optimiser des sondes pour n'importe quel essai fondé sur une hybridation comprenant des jeux ordonnés de microéchantillons, des essais à base de billes des essais de génotypage et des essais à base d'ARNi.

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