Probes and primers for detecting prostate specific antigen...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2360073

Nucleic acid sequences for detecting the presence of nucleic acids, particularly mRNA, encoding human prostate-associated genetic markers encoding prostate-specific antigen (PSA), prostate specific membrane antigen (PSMA) or human kallikrein 2 (hK2) are disclosed. Preferred combinations of nucleic acid sequences amplifying and detecting the prostate-associated genetic markers RNA, used in methods that include amplification of the target sequences and detection of the amplified sequences are disclosed. Methods of detecting the presence of prostate-associated genetic marker nucleic acids, particularly mRNA, in a biological sample of non-prostate origin are disclosed.

L'invention concerne des séquences d'acide nucléique permettant de détecter la présence d'acides nucléiques, notamment un ARN messager, codant pour des marqueurs génétiques humains associés à la prostate codant pour l'antigène prostatique spécifique (PSA), l'antigène membranaire spécifique de la prostate (PSMA) ou la kallicréine humaine (hK2). L'invention concerne également des combinaisons préférées de séquences d'acide nucléique qui amplifient et détectent l'ARN messager des marqueurs génétiques associés à la prostate, lesquelles combinaisons sont utilisées dans des méthodes qui consistent à amplifier les séquences cibles et à détecter les séquences amplifiées. L'invention concerne également des méthodes permettant de détecter la présence, dans un échantillon biologique d'origine non prostatique, d'acides nucléiques de marqueurs génétiques associés à la prostate, notamment un ARN messager.

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