Probes for the diagnosis of infections caused by...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2284555

The DNA from the bacteria Streptococcus pyogenes is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Streptococcus pyogenes, then the sequence of the probe is elucidated.

L'invention a trait à des sondes obtenues par extraction d'ADN dont est porteur Streptococcus pyogenes. Ces ADN extraits sont entièrement digérés par une enzyme de restriction, HindIII, et clonés dans un vecteur approprié. Il est alors procédé à un criblage des sondes contenant les fragments d'ADN essentiellement portés par Streptococcus pyogenes suivi d'une clarification des séquences de base de ces sondes.

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