Probes for the diagnosis of infections caused by klebsiella...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2284858

The DNA from the bacteria Klebsiella pneumoniae is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Klebsiella pneumoniae , then the sequence of the probe is elucidated.

L'invention porte sur des sondes obtenues par: extraction d'ADN de Klebsiella pneumoniae, digestion complète dudit ADN par l'enzyme de restriction HindIII, clonage dudit ADN en un vecteur approprié, et criblage des sondes contenant des fragments d'ADN intrinséquement portés par le Klebsiella pneumoniae suivi de la clarification des séquences de base de ces sondes.

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