Procedure for exposing an epitope within a protein...

Details Procedure for exposing an epitope within a protein... Procedure for exposing an epitope within a protein...

C - Chemistry, Metallurgy

12

N

167/129, 167/37,

C12N 15/70 (2006.01) A61K 47/48 (2006.01) C07K 14/105 (2006.01) C07K 14/245 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 15/64 (2006.01) C12N 15/74 (2006.01) G01N 33/566 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1324333

PROCEDURE FOR EXPOSING AN EPITOPE WITH IN A PROTEIN POSSESSING A DISTINCT POLYPEPTIDE STRUCTURE AND THE PRODUCTS OBTAINED ABSTRACT The invention relates to a procedure for the incorporation of a DNA sequence coding for a peptide immunogen into a DNA vector. This procedure includes the cleavage of the DNA vector at distinct sites, preferably in a random manner, the recombination of the different fragments with the sequence coding for the protein, the transformation of competent cells with the series of the combined fragments, the detection and isolation off those cell colonies which express the products recognized by antibodies to the peptide immunogen.

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