Procedure for the detection of pathogens using dna probes

Details Procedure for the detection of pathogens using dna probes Procedure for the detection of pathogens using dna probes

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/04 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2038742

Abstract: Disclosed are novel methods by which a rapid, multisample, non-radioactive procedure to detect pathogens, such as Plasmodium flaciparum parasites, in biological fluids including human blood samples is achieved. The detection is bases on the use of parasite specific DNA probes and sandwich hybridization technique employing microtitre plates. The high sensitivity and specificity of these assays and the ease with which they can be performed enables on to use them for routine analyses of a large number of blood and other coloured tissue samples of vertebrates and invertebrates. Especially, these assays can be used to detect the presence of P.falciparum. The procedure described is amenable for application in a wide variety of DNA detection analysis using non radioactive DNA probes. In regard to detection of P.falciparum, the invention also relates to novel DNA fragments and hybridisation probes based on such fragments. The invention provides a diagnostic kit on the basis of the novel methods.

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