C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/56 (2006.01) C12Q 1/37 (2006.01) G01N 33/573 (2006.01) G01N 33/86 (2006.01)
Patent
CA 2311479
A procedure for the determination of the activity of the protease which activates blood clotting factor VII from protein solutions, is described in which the protein solution comprising the protease and/or its proenzyme is incubated with a solid phase to which an antibody directed against the protease has been coupled beforehand and after washing the solid phase the protease and/or its proenzyme fixed thereto are incubated with reagents which allow determination of their activity.
L'invention porte sur un protocole permettant de déterminer l'activité de la protéase qui active le facteur VII de la coagulation sanguine présent dans des solutions de protéines. La solution de protéines qui contient la protéase et/ou sa proenzyme est incubée avec une phase solide préalablement couplée à un anticorps dirigé contre la protéase. Puis, après un lavage de la phase solide, la protéase et/ou sa proenzyme fixée à cette dernière est incubée avec des réactifs qui permettent la détermination de leur activité.
Feussner Annette
Roemisch Juergen
Stoehr Hans-Arnold
Aventis Behring Gmbh
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Csl Behring Gmbh
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