Process and a device for improved oxygenation of biological...

C - Chemistry – Metallurgy – 12 – M

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C12M 1/06 (2006.01) B01F 3/04 (2006.01) C12M 3/02 (2006.01) C12N 1/00 (2006.01)

Patent

CA 1242402

ABSTRACT OF THE DISCLOSURE A process is provided for the oxygenation of a biological culture e.g. a mammalian culture. The process comprises carrying out the cultivation in a liquid suspension in a closed vessel under agitation by means of a mechanical stirrer which is in direct contact with the liquid suspension. The oxygen is supplied to the culture through at least one oxygen-permeable membrane carried by the stirrer. The reaction vessel for such cultures comprises a reaction vessel provided with at least one stirrer, the stirrer having inlet conduit means for passing an oxygen-containing gas from the exterior of the vessel through at least one oxygen-permeable membrane and also having separate outlet conduit means for discharging the gas from the at least one membrane to the exterior of the vessel. The side of the stirrer opposite to the inlet conduit means is adapted to be in contact with the culture of the biological material.

467547

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