Process and a reagent for the determination of .alpha.-amylase

Details Process and a reagent for the determination of .alpha.-amylase Process and a reagent for the determination of .alpha.-amylase

C - Chemistry, Metallurgy

12

Q

195/139, 150/15.

C12Q 1/40 (2006.01)

Patent

CA 1120836

ABSTRACT The present invention provides a process for the determination of .alpha.-amylase by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein, as substrate, there is used a compound of the general formula :- Image (I) in which R is a glucoside, phenylglucoside, mononitro- phenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. The present invention also provides a reagent for the determination of .alpha.-amylase, comprising an .alpha.- amylase substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, wherein the substrate is a compound of the general formula (I) given above.

309495

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