G - Physics – 01 – N
Patent
G - Physics
01
N
150/14, 150/3
G01N 33/72 (2006.01)
Patent
CA 1211348
Abstract The present invention provides a process for the determination of direct and total bilirubin in body fluids in which the bilirubin is coupled with a diazonium salt and the extinction change thereby brought about is measured according to known methods, wherein the coupling is carried out in the presence of a buffer which adjusts the test solution to a weakly acidic to approximately neutral pH value and, as coupling component, there is used a diazonium salt of the general formula:- Image (I), in which R1 is a hydrogen or halogen atom or a lower alkyl, lower alkoxy or benzoylamino radical, R2 is a hydrogen atom or a lower alkyl or lower alkoxy radical, R3 is a hydrogen or halogen atom or a lower alkyl or lower alkoxy radical, whereby R1 and R3 do not simult- aneously represent halogen atoms, and X is an anion forming a storage-stable salt with the diazonium cation. The present invention also provides an agent for carrying out this process which comprises a diazonium salt of general formula (I) and an appropriate buffer.
431532
Hoffmann Georg-Erich
Weiss Ludwig
Boehringer Mannheim G.m.b.h.
Marcoux & Sher Swabey Mitchell Houle
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