Process and agent for the detection of an analyte

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/52 (2006.01) G01N 33/74 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1293187

ABSTRACT Process and agent for the detection of an analyte The present invention provides a process for the detection of an analyte, wherein a sample containing the analyte is incubated either with a labelled binding partner and the immobilised analyte or an immobilised analyte analogue or with the labelled analyte or analyte analogue and an immobilised binding partner, the binding partner displaying towards the immobilised or labelled analyte or analyte analogue a higher affinity than towards the free analyte, and the labelled binding partner or the labelled analyte of the labelled analyte analogue is present in insufficiency compared with the concentration of the analyte to be detected. The present invention also provides an agent for carrying out this process, wherein it contains either the immobilised analyte or an immobilised analyte analogue and a labelled binding partner or the labelled analyte or a labelled analyte analogue and an immobilised binding partner, as well as optionally an appropriate buffer system and further conventionally used adjuvants, the binding partner displaying towards the immobilised or labelled analyte or analyte analogue a higher affinity than towards the free analyte.

542067

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