Process and apparatus for contactless micro injection,...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/24 (2006.01) B01L 3/02 (2006.01) C12M 1/26 (2006.01) C12N 1/02 (2006.01) C12N 5/02 (2006.01) C12N 15/89 (2006.01) G01N 1/04 (2006.01) G01N 1/28 (2006.01) G01N 1/31 (2006.01) G01N 15/14 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2245553

The invention concerns a method and device for the contactless microinjection, sorting and production of biological objects. According to the invention, on a planar carrier (2) an object field or the object itself, located on the carrier (2), is cut out with a laser beam (6) and transferred by means of a laser-induced transport process to a collector substrate (5) which is disposed directly above or below the carrier. During the cutting-out process, either the laser beam (6) moves in a closed curve about the object or the object itself is cut directly out of the carrier (2) in a computerized manner. This method enables individually selected objects to be spatially separated and sorted from a very large number of objects. The method can also be used to separate specific cells from tissue sections. The method is further suitable for microinjecting given substances into individual biological objects, such as for example cells, and then sorting the latter.

L'invention concerne un procédé et un dispositif de micro-injection sans contact, de tri et de production d'objets biologiques. Sur un support (2) planaire, un champ d'objet ou un objet situé sur le support (2) est découpé à l'aide d'un faisceau laser (6) et est transféré par processus de transport induit par laser, sur un substrat collecteur (5) placé immédiatement au-dessus ou en dessous du support. Lors du processus de découpage, l'objet est soit contourné par le faisceau laser (6) en une courbe fermée ou est découpé directement à partir du support (2), de manière assistée par ordinateur. Ce procédé permet de séparer et de trier dans l'espace des objets individuels sélectionnés parmi un très grand nombre d'objets. Ce procédé peut également s'utiliser pour séparer des cellules spécifiques dans des coupes tissulaires. Ce procédé s'utilise en outre pour effectuer des micro-injections de substances déterminées dans des objets biologiques individuels, par ex. des cellules, puis les extraire.

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