Process and apparatus for the genetic transformation of cells

Details Process and apparatus for the genetic transformation of cells Process and apparatus for the genetic transformation of cells

C - Chemistry, Metallurgy

12

N

195/141, 195/1.3

C12N 15/87 (2006.01) C12M 3/00 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12N 15/89 (2006.01)

Patent

CA 2032443

GA/57-17879/=/ETH Process and Apparatus for the Genetic Transformation of Cells ABSTRACT In a tubular pressure chamber a drop of a DNA solution with gold particles suspended therein is atomized at the opening of a cannula by a pressure impact. The fog droplets containing the gold particles and the DNA entrained by them, are pressured by the pressure impact through a restriction at the end of the pressure chamber and thereby accelerated and focused. They subsequently traverse in free flight an evacuated specimen chamber and impact in a narrowly limited target area with a predetermined pulse, cells fixed on a holder, thereby penetrating them.

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