Process and composition for determining the activity of...

G - Physics – 01 – N

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167/139, 167/37

G01N 33/48 (2006.01) A61K 37/00 (1980.01)

Patent

CA 1062609

ABSTRACT OF THE DISCLOSURE A method, composition and test kit are provided for determination of CK-MB activity in a sample of biological liquids. The method comprises the steps of incubating the sample, optionally in the presence of CK substrates, with antibodies which inhibit enzymatic activity of the M subunit in CX isoenzymes MM and MB without substantially inactivating enzymatic activity of the B subunit in any CK-MB which may be present, and then determining the activity of the CK B subunit in the resultant sample. This provides a simple, rapid and reproducible method for the diagnosis of cardiac infarction.

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