Process and compositions for preparing phenylalanine

C - Chemistry – Metallurgy – 12 – P

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195/134.1, 195/3

C12P 13/22 (2006.01) C12N 9/10 (2006.01) C12N 11/02 (2006.01)

Patent

CA 1276900

PROCESS AND COMPOSITIONS FOR PREPARING PHENYLALANINE ABSTRACT OF THE DISCLOSURE A process for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having transaminase activity in the presence of an amine donor. Ruptured or permeabilized cells, with the enzyme in the free or immobilized state, may also be used. The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also disclosed. Improvement in yield by operating at a Keg of greater than 5 is also disclosed.

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