G - Physics – 01 – N
Patent
G - Physics
01
N
150/11
G01N 33/48 (2006.01) G01N 27/416 (2006.01) G01N 33/487 (2006.01) G01N 33/72 (2006.01) G01N 33/80 (2006.01) G01N 33/86 (2006.01) G01N 33/96 (2006.01)
Patent
CA 1086616
METHOD AND APPARATUS FOR THE CONTINUOUS DETERMINATION OF THE CONCENTRATION OF AN ENZYME SUBSTRATE ABSTRACT OF THE DISCLOSURE Enzyme substrates in aqueous liquids are continuously measured by introducing a sample containing the substrate to be determined into a current of buffer solution, reacting the sub- strate on an immobilized enzyme and measuring, in a measurement chamber, a physical change in the solution brought about by the reaction, wherein a current of buffer solution containing the sample to be determined is passed alternatingly (i) directly through the measurement chamber with the production of a refer- ence signal and (ii) first over the immobilized enzyme and thereafter through the measurement chamber with the production of a measurement signal, and relating the reference signal to the measurement signal to indicate the enzyme substrate initially present.
283810
Edelmann Hermann
Hagen Alexander
Kraft Peter
Mann Karlheinz
Schuler Peter
Boehringer Mannheim G.m.b.h.
Sherman
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