Process and method for monitoring gastrointestinal microbiota

Details Process and method for monitoring gastrointestinal microbiota Process and method for monitoring gastrointestinal microbiota

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2720292

Disclosed are methods for monitoring the gastrointestinal tract of the human gastrointestinal system. The method includes: 1) grouping microbes into specific operational taxonomic units (OTU); 2) using oligonucleotide probes and PCR primers to detect and quantify specific microbes (bacteria, fungi/yeast, protozoans and parasitic worms) in human fecal material. The inventions also discloses a kit that includes: a DNA isolation step; 2) accumulation of specific operational taxonomic units (OTU); 3) identification and quantifying of sequences internal to the OTU; 4) reporting changes the indigenous population of the human gastrointestinal system.

L'invention porte sur des procédés pour surveiller le tractus gastro-intestinal du système gastro-intestinal humain. Le procédé comprend les opérations consistant à : 1) grouper des microbes en unités taxonomiques opérationnelles spécifiques (OTU) ; 2) utiliser des sondes oligonucléotidiques et des amorces de PCR pour détecter et quantifier des microbes spécifiques (bactéries, champignons/levure, protozoaires et vers parasites) dans une matière fécale humaine. Les inventions décrivent également un kit qui comprend : une étape d'isolation de l'ADN ; 2) une accumulation d'unités taxonomiques opérationnelles spécifiques (OTU) ; 3) une identification et une quantification de séquences internes à l'OTU ; 4) le rapport de changements de la population indigène du système gastro-intestinal humain.

Affiliated with

Also associated with

Rating

Process and method for monitoring gastrointestinal microbiota does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process and method for monitoring gastrointestinal microbiota, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process and method for monitoring gastrointestinal microbiota will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1959672