Process and reagent for determination of uric acid

Details Process and reagent for determination of uric acid Process and reagent for determination of uric acid

C - Chemistry, Metallurgy

12

Q

150/15, 150/3.3

C12Q 1/00 (2006.01) C12Q 1/62 (2006.01)

Patent

CA 1098013

ABSTRACT OF THE DISCLOSURE Uric acid in biological material is determined by means of uricase, catalase and aldehyde dehydrogenase in the presence of alcohol and nicotinamide-adenine-dinucleotide or phosphate (NAD(P), and at least one compound selected from oxalates, malonic acid mono(lower) alkyl esters, trihalo- ethanols, pyrazole and pyridine optionally substituted with one or more lower alkyl radicals and/or halogen atoms, pyridine-carboxylic acids optionally substituted by a lower alkyl radical and the amides and lower alkyl esters thereof, thiourea, isobutyramide and chelate-forming complexing agents; in this way there is provided a disturbance-free determination of uric acid in, for example, serum and plasma, which may be important in, for example, the clinical investigation of gout.

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