G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/78 (2006.01)
Patent
CA 2000545
The present invention provides a process for the determination of a specifically bindable substance by incubation of a sample solution with at least three receptors R1, R2 and R3, of which R1 is specifically bindable with R2 and R3, as well as with the substance to be determined, R2 brings about the binding to a solid phase and R3 carries a labelling, separation of bound labelling from unbound labelling and measurement of the labelling in one of: the two phases, wherein, as receptor R1, a receptor is used which has at least two binding positions which bind specifically with an epitope of the substance to be determined, as R2 a conjugate of a partner P1 of a specifically binding pair and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, the partner Pl thereby either being bound to a solid phase or being immobilized, and as R3 a complex is used which contains at least the substance S and a labelling. The present invention also provides a reagent for carrying out this process.
Boehringer Mannheim Gmbh
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Roche Diagnostics Gmbh
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