Process and reagent for the determination of an oxidized...

Details Process and reagent for the determination of an oxidized... Process and reagent for the determination of an oxidized...

C - Chemistry, Metallurgy

12

Q

150/15, 150/3.2

C12Q 1/66 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 1131109

ABSTRACT OF THE DISCLOSURE Process and reagent for the quantitative determination of an oxidised pyridine co-enzyme with oxidation with oxygen in the presence of the luciferase system and measurement of the emitted light, wherein an oxidised pyridine co-enzyme is reacted in the presence of its specific alcohol dehydro- genase with an aliphatic alcohol containing 8 to 16 carbon atoms and the aldehyde formed is oxidised with reduced flavine mononucleotide (FMNH2) and oxygen in the presence of luciferase and of the particular FMN reductase which is independent of the co-enzyme to be determined, as well as of the reduced substrate co-enzyme of the latter, with light emission the reagent comprises luciferase, FMN reductase, the aliphatic alcohol of 8 to 16 carbon atoms, the reduced co-enzyme, the alcohol dehydrogenase specific for the co-enzyme to be determined, the flavine-mononucleotide (FMN), a buffer and optionally an organic sulphhydryl compound and/or a surface- active agent.

332917

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