Process and reagent for the determination of cholesterol

Details Process and reagent for the determination of cholesterol Process and reagent for the determination of cholesterol

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/28 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 1179243

ABSTRACT The present invention provides a process for the determination of cholesterol in free or found form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol. The present invention also provides a reagent for the kinetic determination of cholesterol, com- prising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro- organism of the genus Streptomyces.

390371

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