Process and reagent for the enzymatic determination of...

Details Process and reagent for the enzymatic determination of... Process and reagent for the enzymatic determination of...

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/26 (2006.01) C12Q 1/54 (2006.01) C12Q 1/60 (2006.01) C12Q 1/62 (2006.01)

Patent

CA 1152871

ABSTRACT The present invention provides a reagent for the enzymatic determination of an enzyme substrate, contain- ing a system for the determination of the substrate, which comprises at least one enzyms, at least one buffer substance and at least one indicator substance and optionally contains adjuvants, wherein the reagent con- tains a definite amount of the enzyme substrate in an insufficient amount with regard to the substrate deter- mination capacity of the system for the determination of this substrate. The present invention also provides a process for the enzymatic determination of an enzyme substrate in the presence of disturbing substance which can react with the substrate itself or with an intermediate or end product of the indicator reaction, wherein, before the addition of the sample to be investigated, a definite amount of the substrate is first reacted with the reagent until reaction is complete, whereafter the sample to be determined is added.

347407

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