Process and reagent for the enzymatic determination of sulphite

Details Process and reagent for the enzymatic determination of sulphite Process and reagent for the enzymatic determination of sulphite

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/28 (2006.01) C12Q 1/26 (2006.01) G01N 33/02 (2006.01)

Patent

CA 1223801

ABSTRACT Process and reagent for the enzymatic determination of sulphite The present invention provides a process for the enzymatic determination of sulphite, wherein the sulphite is oxidised in a buffered solution with sulphite oxidase to give sulphate, the hydrogen peroxide thereby formed being reduced in the presence of NAD and NADH with the enzyme NADH peroxidase and the consumption of NADH being determined optically. The present invention also provides a reagent for the enzymatic determination of sulphite, wherein it contains sulphite oxidase, NADH, NAD, NADH peroxidase and buffer.

451582

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