G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/577 (2006.01) C07K 16/40 (2006.01) C12Q 1/40 (2006.01)
Patent
CA 1276106
ABSTRACT Process and reagent for the specific determination of pancreatic alpha-amylase The present invention provides a process for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, espec- ially in serum, plasma, duodenal juice or urine, by reaction with a system for the detection of alpha- amylase in the presence of a monoclonal antibody which inhibits salivary alpha-amylase / wherein, as inhibitor, there is used a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal anti- saliva alpha-amylase antibody which inhibits this enzyme by less than 10%. The present invention also provides a reagent for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, duodenal juice, plasma or urine, containing a system for the detection of alpha-amylase and a monoclonal antibody against salivary alpha-amylase wherein it contains a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal anti-salivary alpha-amylase antibody which inhibits this enzyme by less than 10%.
514128
Albert Winfried
Gerber Martin
Lenz Helmut
Boehringer Mannheim Gmbh
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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