G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 150/26,
G01N 33/52 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) G01N 21/78 (2006.01) G01N 33/66 (2006.01)
Patent
CA 1298179
ABSTRACT Process and reagent for the specific determination of fructosamine The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate colour reagent and measurement of the colour change thereby brought about, wherein, before the colour reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the colour reagent is added thereto. The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity- causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a colour reagent for the detection of fructosamine.
540108
Schellong Lieselotte
Siedel Joachim
Vogt Bernd
Ziegenhorn Joachim
Boehringer Mannheim Gmbh
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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