Process and reagent for the specific determination of...

C - Chemistry – Metallurgy – 07 – H

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C07H 15/203 (2006.01) C07H 17/00 (2006.01) C12P 19/18 (2006.01) C12Q 1/40 (2006.01) G01N 33/573 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2023801

ABSTRACT Process and reagent for the specific determination of pancreatic .alpha.-amylase The present invention provides a process for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids by reaction with a system for the detection of .alpha.-amylase with the use of an inhibitor for salivary .alpha.-amylase, wherein, as substrate, there is used a compound of the general formula:- Image (I) in which R1 is a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms, a cycloalkyl or cycloalkoxyl radical containing 3 to 6 carbon atoms or a benzoyl, benzyl or phenyl radical which is optionally hydrophilically substituted, R2 is a hydrogen atom or in which R1 and R2 together form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, Gluc is a glucose molecule, n is 1, 2 or 3 and X is an optically determinable residue. The present invention also provides a reagent for the specific determination of pancreatic .alpha.-amylase, as well as new compounds for use in the above process and reagent.

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