C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.2, 150/3.
C12Q 1/44 (2006.01) C12Q 1/60 (2006.01) G01N 33/92 (2006.01)
Patent
CA 1309645
ABSTRACT Process and reagent for the specific determination of the cholesterol of HDL fraction The present invention provides a process for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of the lipoproteins of the serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and kinetic measurement of the hydrogen peroxide formation or of the oxygen consumption, wherein the measurement is carried out within 2 to 15 minutes after the start of the oxidase reaction at a temperature of from 20 to 40°C. during a predetermined time interval and during the measurement there is maintained in the reaction solution a cholesterol esterase concentration of 0.05 to 30 U/ml., a cholesterol oxidase concentration of 0.1 to 50 U/ml., a concentration of a tenside of the bile acid group of 1.0 to 20 mMole/litre, a concentration of a non-ionic detergent of 0.1 to 10 g./litre and a pH value of 5 to 9. The present invention also provides a reagent for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of the lipoproteins of the serum which reagent contains cholesterol esterase, cholesterol oxidase, a tenside of
549035
Kerscher Lorenz
Pautz Brigitte
Trunk Gisela
Ziegenhorn Joachim
Boehringer Mannheim Gmbh
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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