Process for adsorption-based separation of bioparticles from...

B - Operations – Transporting – 01 – D

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B01D 15/08 (2006.01) B01J 20/28 (2006.01)

Patent

CA 2608340

A process for adsorption-based separation of bioparticles from an aqueous suspension containing such bioparticles is disclosed which process comprises a) bringing said aqueous suspension into contact with a macroporous cryogel monolith carrying on its surface a ligand capable of interacting specifically or non-specifically with a receptor on the surface of a bioparticle to be separated to adsorb said bioparticle to said macroporous cryogel monolith; and b) liberating said bioparticle from said macroporous cryogel monolith by elution, wherein said macroporous cryogel monolith is subjected to a physical deformation in con-junction with exposure to eluting medium.

La présente invention concerne un processus de séparation par adsorption de bioparticules contenues dans une suspension aqueuse qui consiste à: a) mettre ladite suspension aqueuse en contact avec un bloc de cryogel macroporeux portant sur sa surface un ligand capable d'interagir spécifiquement ou non spécifiquement avec un récepteur situé sur la surface d'une bioparticule devant être séparée afin d'adsorber la bioparticule sur le bloc de cryogel macroporeux; et b) libérer par élution, ladite bioparticule du bloc de cryogel macroporeux, ledit bloc de cryogel macroporeux étant soumis à une déformation physique conjointement à l'exposition au milieu d'élution.

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