Process for allele discrimation utilizing primer extension

Details Process for allele discrimation utilizing primer extension Process for allele discrimation utilizing primer extension

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2405687

Disclosed are methods for allele discrimination involving the use of rolling circle amplification (RCA) coupled with primer extension and utilizing exonuclease deficient polymerases to distinguish matched and unmated single nucleotide sites, such as in the case of a single nucleotide polymorphism (SNP).

L'invention se rapporte à des procédés de discrimination d'allèles consistant à mettre en oeuvre une amplification par cercle roulant (RCA) conjointement à un allongement d'amorce et à utiliser des polymérases dépourvues d'activité d'exonucléase pour distinguer les sites de nucléotides simples appariés et non appariés, comme par exemple dans le cas d'un polymorphisme mononucléotidique (SNP).

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