C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
195/122, 195/1.1
C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 1340121
The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired. In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.
617031
Erlich Henry A.
Horn Glenn T.
Mullis Kary B.
Saiki Randall K.
Scharf Stephen J.
Borden Ladner Gervais Llp
F. Hoffmann-La Roche
F. Hoffmann-La Roche Ag
LandOfFree
Process for amplifying, detecting and/or cloning nucleic... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for amplifying, detecting and/or cloning nucleic..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for amplifying, detecting and/or cloning nucleic... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1263483