Process for attaching effector molecules to proteins

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/107 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07K 16/00 (2006.01) C07K 17/08 (2006.01) C08G 65/334 (2006.01)

Patent

CA 2613481

The present invention provides a process for attaching one or more effector molecules to one or more cysteines in a protein comprising: a) activating one or more cysteines in the protein by diafiltering the protein against a monothiol reducing agent or a multi-thiol reducing agent which is incapable of forming intramolecular disulphide bonds and b) reacting the treated protein with an effector molecule.

Procédé de fixation d'une ou plusieurs molécules effectrices sur une ou plusieurs cystéines dans une protéine : a) activation de la ou des cystéines dans la protéine par diafiltration de la protéine par rapport à un agent réducteur monothiol ou multithiol incapable de former des liaisons désulfure intramoléculaires et b) réaction de la protéine traitée avec une molécule effectrice..

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