C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/06 (2006.01)
Patent
CA 2557046
The invention relates to a process for the culturing of cells by continuous perfusion culturing of a cell culture comprising cell culture medium and cells, wherein cell culture medium is added to the cell culture, the cell culture is circulated over a filter module comprising hollow fibers resulting in an outflow of liquid having a lower cell density than the cell culture and the flow within the filter module is an alternating tangential flow. Preferably, culture medium is added at a particular perfusion rate and/or biomass is removed form the culture at least once. The method is especially suitable for the culturing of aggregating cells. The invention also relates to such a process wherein a biological substance, preferably an antibody, is produced by the cells, which biological substance may be further purified in downstream processing.
L'invention a trait à un procédé permettant de cultiver des cellules par la mise en culture par perfusion continue d'une culture cellulaire contenant un milieu de culture cellulaire et des cellules. Le procédé selon l'invention consiste à ajouter le milieu de culture cellulaire à la culture cellulaire, et à faire passer la culture cellulaire par un module filtrant comportant des fibres creuses, ce qui génère un flux de liquide présentant une densité cellulaire inférieure à celle de la culture cellulaire, le flux dans le module filtrant étant un flux tangentiel alternatif. Le milieu de culture est de préférence ajouté à une vitesse de perfusion particulière, et/ou la biomasse est éliminée de la culture au moins une fois. Le procédé selon l'invention convient particulièrement à la mise en culture de cellules agrégées. L'invention concerne également un procédé selon lequel une substance biologique, de préférence un anticorps, est produite par les cellules, ladite substance biologique pouvant être purifiée au cours d'un traitement en aval.
Coco Martin Jose Manuel
Crowley John
Wuebben Maike
Dsm Ip Assets B.v.
Smart & Biggar
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