Process for cell proliferation

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A61F 2/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2656984

In vitro process for the proliferation or culture of conjunctive tissue cells selected from the group consisting of chondrocytes, osteoblasts, chondroprogenitor cells, osteoprogenitor cells, tenocytes and ligament cells comprising the step of contacting said cells with an aminosugar selected from the group consisting of mannosamine, N-acetylmannosamine, mannosamine salts and their mixtures. The present invention also relates to compositions which comprise the aminosugar in combination with the cells. These compositions are useful for the treatment of cartilage, bone, tendon and ligament lesions or defects, bone mass losses and osteochondral defects or lesions.

La présente invention concerne un procédé de prolifération ou de culture in vitro de cellules de tissu conjonctifs choisies parmi le groupe constitué de chondrocytes, d'ostéoblastes, de cellules souches chondrocytaires, de cellules souches ostéoblastiques, de cellules ténocytes et ligamentaires comprenant l'étape de mise en contact desdites cellules avec un sucre aminé choisi parmi le groupe constitué de mannosamine, N-acétylmannosamine, de sels mannosamine et leurs mélanges. La présente invention concerne également des compositions comportant le sucre aminé en combinaison avec les cellules. Ces compositions sont utiles pour le traitement de lésions et de défectuosités de cartilage, d'os, de tendon et de ligament, de perte de masse osseuse et de défectuosités ou lésions ostéochondrales.

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