Process for cell surface phenotyping of lymphocyte...

Details Process for cell surface phenotyping of lymphocyte... Process for cell surface phenotyping of lymphocyte...

G - Physics

01

N

167/37

G01N 33/545 (2006.01) G01N 33/80 (2006.01)

Patent

CA 1211047

Abstract of the Invention Cell surface phenotyping of lymphocyte subpopulations using polystyrene plates having a plurality of small wells is accomplished by introducing to the wells antibody molecules specific for cell-surface antigens, which adhere to or are absorbed to the well surfaces, introducing to the wells a predetermined quantity of peripheral blood lymphocyte cells, incubating the cells in the presence of a cell nutrient, removing nonadherent cells, introducing different antibodies to different wells, introducing complement to the wells, and determining the degree of lyis of the cells.

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