C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/74 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2665120
The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
La présente invention se rapporte à un nouveau procédé de remplacement ou de délétion de séquences d'ADN dans Clostridia, se révélant très efficace, facile à mettre en AEuvre et applicable au niveau industriel. Ce procédé peut être utilisé pour modifier plusieurs locus génétiques dans Clostridia de façon routinière. Ce procédé est fondé sur un vecteur réplicatif porteur d'au moins deux gènes marqueurs.
Croux Christian
Figge Rainer
Soucaille Philippe
Gowling Lafleur Henderson Llp
Metabolic Explorer
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