Process for cross-linking cellulose ester membranes

Details Process for cross-linking cellulose ester membranes Process for cross-linking cellulose ester membranes

B - Operations, Transporting

01

D

B01D 71/14 (2006.01) B01D 67/00 (2006.01) B01J 20/00 (2006.01) B01J 20/32 (2006.01) C08B 15/00 (2006.01)

Patent

CA 2616867

The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.

L'invention concerne un procédé permettant de rendre des membranes de cellulose réticulées poreuses et des procédés permettant de coupler un ligand chromatographique à des membranes de cellulose réticulées. L'invention concerne également des procédés permettant de séparer un premier composant d'un second composant dans une solution basée sur la différence entre la dimension des premier et second composants, et des procédés permettant de séparer des molécules cibles d'autres composants dans une solution qui consistent à utiliser les membranes obtenues au moyen du procédé de l'invention. Ledit procédé présente une utilité particulière pour séparer des protéines de lysats et de cultures cellulaires.

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