Process for cultivating bacillus thuringiensis biopesticides...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) A01N 63/00 (2006.01) C02F 11/02 (2006.01)

Patent

CA 2193378

Disclosed herein is a process for preparing a Bacillus thuringiensis bio- insecticide, the process comprising the steps of: (a) inoculating wastewater treatment plant sludge(s) with Bacillus thuringiensis bacteria; (b) aerobically cultivating the bacteria in the sludge(s) for a period of time sufficient for producing the bio-insecticide resulting from the sporulation of the bacteria through synthesis of insecticidal .delta.-endotoxin proteins in the form of parasporal crystals; (c) recovering the sludge(s) containing the resulting bio-insecticide.

On décrit ici un procédé de préparation d'insecticides biologiques produits par Bacillus thuringiensis. Ce procédé consiste à: (a) inoculer une boue provenant d'une installation de traitement d'eaux usées avec la bactérie Bacillus thuringiensis; (b) réaliser une culture aérobie de cette bactérie sur la boue, pendant une durée suffisante pour obtenir un insecticide biologique suite à la sporulation de la bactérie par synthèse des .delta.-endotoxines protéiques insecticides sous la forme de cristaux parasporaux; et à (c) récupérer la boue contenant l'insecticide biologique en question.

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