Process for decreasing aggregate levels of pegylated protein

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/18 (2006.01) C07K 1/22 (2006.01) C07K 1/32 (2006.01) C07K 14/61 (2006.01)

Patent

CA 2498886

The present invention is directed generally to recombinant methods for making a desired pegylated protein and pooling of same. These method(s) yield a polypeptide product containing reduced levels of aggregate thereof pooled to provide the desired pegylated isoforms thereof.

L'invention concerne, de façon générale, des procédés de recombinaison servant à produire une protéine PEGylée et à en effectuer le regroupement. Ces procédés permettent d'obtenir un polypeptide contenant des niveaux limités d'agrégat de ladite protéine regroupés de manière à produire les isoformes PEGylées de ladite protéine.

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